Coding

Part:BBa_K4345016

Designed by: Paula Ventura   Group: iGEM22_KU_Leuven   (2022-09-30)


ccdB with STOP codon control

CcdB protein is toxin of type II toxin-antitoxin system CcdB/CcdA. CcdB is a toxin that kills E. coli by targeting the GyrA subunit of DNA gyrase, an essential type II topoisomerase (Bahassi et al., 1999). In this part, we added three stop codons, one in each reading frame, to prevent the translation and be able to clone CcdB into our host. The stop codons are flanked by SalI restriction sites.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 249
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Bahassi, E. M., O’Dea, M. H., Allali, N., Messens, J., Gellert, M., & Couturier, M. (1999, April). Interactions of CcdB with DNA Gyrase. Journal of Biological Chemistry, 274(16), 10936–10944. https://doi.org/10.1074/jbc.274.16.10936

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Categories
Parameters
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